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Encompassing Amino Acids: 2-403
Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: The assay was performed by UV absorption using a recombinant IDO1 or TDO2 and L-Tryptophan substrate. The UV absorption signal at 321 nm is correlated with the amount of N-formylkynurenine reaction product of reaction. All of the reactions were conducted at room temperature. The 100 µl reaction mixture in IDO or TDO Assay Buffer contains ~40 nM IDO1 (200 ng/well for 71182 or 210 ng/well for 79081) or varying concentrations (0-320 nM) of IDO1, 900 tryptophan, and the coupled reaction components. The reaction mixture incubated for 165 min respectively while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals were measured using a Tecan Infinite M1000 plate reader.
Description: Human IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_002164, a.a. 2-403(end) with an N-terminal His-Avi-Tag, expressed in an E. coli cell expression system. MW = 48 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 20% glycerol
Genbank: NM_002164
Storage Stability: At least 6 months at -80°C.
Tags: His-Avi-Tag
Uniprot: P14902
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157.2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530.3. Flick, H.E., et al., Int. Nat. J. of Tryptophan Res. 2013; 6: 35-45.
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