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Encompassing Amino Acids: Full Length
Applications: Useful for studying enzyme kinetics and screening small molecule inhibitors.
Assay Conditions: Assay was done in a Kinase buffer using IKKtide as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human IKKβ, also known as IKK2, IKBKB, IKKB, and NFKBIKB. GenBank Accession No. NM_001556, full-lengthexpressed in a baculovirus-infected Sf9 insect cell expression system, MW = 105 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 3 mM DTTand 20% glycerol.
Genbank: NM_001556
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q14920
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Woronicz, J.D. et al., Science. 1997; 278: 866-869.
2. Yin, M.J., et al., Nature. 1998; 396: 77-80.