Application Note: Interleukin-1beta (IL-1β) has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1beta (IL-1β) in immunological assays.
Concentration Value: 0.1 mg/ml
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Biological Activity: IL-1ß activity is determined by the stimulation of D10S.G4.1 cells and is typically less than 12 pg/mL. Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
Physical State: Lyophilized
Purity and Specificity: Recombinant protein corresponding to amino acids 1 to 153 of mature human Interleukin-1beta (IL-1β). Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and by analytical RP-HPLC, each against a known standard.
Background: Interleukin-1beta (IL-1β) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendric cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1β is a non-glycosylated single chain 17,376 Da protein containing 153 amino acid residues.
Low Endotoxin: Yes
Other: User Optimized
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