Products from BPS Bioscience require a minimum order value above 400€
Applications: This kit is useful for cytokine detection in cell culture medium.
Description: Interleukin-10 is a key immunoregulator during various infections that acts as an anti-inflammatory cytokine by inhibiting the activities of Th1, macrophage and NK cells. The IL-10 (Human) Colorimetric ELISA Detection Kit is designed for detecting and quantifying human interleukin-10 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-10 are incubated on the coated plate followed by detecting the captured IL-10 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with capturing and detection antibodies for IL-10, streptavidin-labeled HRP, blocking buffer, IL-10 standard, and colorimetric HRP substrate for a 96-well plate.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Couper, K. N., et al. IL-10: The Master Regulator of Immunity to Infection. J. Immunology, 2008,
180(9): 5771-5777.
English
Deutsch
