Application Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.
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Other Performance Data: Biological Activity: IL-17F activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts and is typically 10-20 ng/mL. Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
Physical State: Lyophilized
Purity and Specificity: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Background: Recombinant Rat IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 133 amino acids and having a molecular mass of 14.9 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A.
Low Endotoxin: Yes
Other: User Optimized
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