In Vitro Biotinylation Kit (with BirA tagless enzyme)

Products from BPS Bioscience require a minimum order value above 400€

Application: Specific biotin labeling of proteins/peptides containing the Avi-Tag™ sequence for downstream applications.

Background: Addition of biotin molecules is commonly used for the labeling and purification of proteins or other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (also known as vitamin H) with avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions known between a protein and a ligand, exhibiting a dissociation constant (Kd) around 4x10^-14 M. BirA, an E. coli biotin protein ligase, covalently adds biotin to an AviTag™ peptide in a highly efficient and specific manner, in a reaction that requires ATP. It has the advantage of resulting in a homogeneous product that can be used in protein capture, immobilization, and functionalization. For more information on enzymatic biotinylation please refer to Enzymatic Biotinylation & BirA (bpsbioscience.com).

Contraindications: The buffer of the protein of interest should not contain ammonium sulfate, as this component inhibits BirA activity. If present, remove ammonium sulfate by dialysis, or spin columns, to Tris, pH 8.0/ NaCl/ glycerol.

Description: The In Vitro Biotinylation Kit (with BirA Tagless Enzyme) is a kit designed for in vitro addition of biotin to biotin-acceptor peptides using BirA. This kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin. The BirA protein provided is a tagless recombinant biotin protein ligase (EC6.3.4.15). It efficiently catalyzes the specific attachment of biotin to the AviTag™. It is a robust reaction in a wide range of conditions, such as 20-40 µM of protein substrate (biotin-acceptor peptide) concentrations and can tolerate up to 1M NaCl and 20% glycerol in the reaction.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• SKU: BPS82511
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82511
• Package Unit: 1 kit
• Quantity Unit: PAK