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Encompassing Amino Acids: 999-end
Applications: Useful for the study of enzyme kinetics, autophosphorylation and phosphorylation of target substrates, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 µM of ATP. Substrate : 4 µM Tyr peptide 1 from Invitrogen.
Description: Human insulin receptor (INSR) (GenBank Accession No. NM_001079817), a.a. 999-end with N-terminal GST-tag, MW=67 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
Genbank: NM_001079817
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P06213
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhou, P. et al. J. Pediatr. Endocrinol. Metab. 21 (4), 369-375 (2008).
2. Machorro-Lazo, M.V. et al., Gynecol. Obstet. Invest. 67 (1), 14-19 (2008).