Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: IRAKs (interleukin receptor associated kinases) are a family of Serine/Threonine kinases that induce a protective acute inflammation response, as well as an adaptive immune response in the presence of pathogens. They are associated with Toll-like receptors in immune cells and transduce an intracellular signaling cascade that results in the transcription of genes involved in inflammation. Four IRAK-like molecules have been identified IRAK1, IRAK2, IRAK4 and IRAKM. IRAK1 and IRAK4 are active kinases, while IRAK2 and IRAKM are inactive. All four kinases mediate activation of NFκB (nuclear factor kappa B), a regulator of innate immunity through signaling pathways that link pathogenic and cellular danger signals in response to invading pathogens. IRAKs have the potential to be used as therapeutic targets due to their highly functional role as mediators of immune response.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The IRAK4 Kinase Assay Kit is designed to measure IRAK4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant IRAK4 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Uniprot: Q9NWZ3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Janssens, S., et al,. Molecular Cell, (2003). 11(2), 293-302.
English
Deutsch
