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Encompassing Amino Acids: 352-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200 µM ATP, 2 µM Tyr1 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.
Description: Human ITK (GenBank Accession No. NM_005546), a.a. 352-end, with N-terminal GST-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.37 mM KCl, 0.05% Tween-20, 30% glycerol, 10 mM glutathione, and 3 mM DTT.
Genbank: NM_005546
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q08881
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. August, A. et al. Int J Biochem Cell Biol. 2002 Oct,34(10):1184-9.
2.Finkelstein, L D. et al. Trends Cell Biol. 2004 Aug,14(8):443-51.