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Encompassing Amino Acids: 808-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Description: Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_004972
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: O60674
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).