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Encompassing Amino Acids: 2-1544(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human JARID1B, also known as KDM5B and PLU-1 (GenBank Accession No. NM_006618), a.a. 2-1544(end) with N-terminal FLAG-Avi-tag, MW=179 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 µg/ml FLAG peptide, and0.2 mM TCEP
Genbank: NM_006618
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-Avi-tag
Uniprot: Q9UGL1
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Yamane K, et al. (2007). Mol. Cell 25 (6):801-12.
2. Barrett A, et al. (2007). Int. J. Cancer 121(2): 265-75.