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Encompassing Amino Acids: 2-1560(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1C (50 - 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, GenBank Accession No. NM_004187, full length, a.a. 2-1560(end), with N-terminal FLAG- and His-tags, MW= 181 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
Genbank: NM_004187
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P41229
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Xu, J., et al. (2008). PLoS One 3(7):e2553
2. Iwase, S., et al. (2007). Cell 128(6):1077-88.
3. Jenson, L.R., et al. (2005). Am. J. Hum. Genet. 76(2):227-36.
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