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Encompassing Amino Acids: 2-186
Applications: Useful for KRAS(G12D) GDP/GTP nucleotide exchange reaction.
Assay Conditions: Assay was performed by following the KRAS Nucleotide Exchange Assay Kit (BPS Bioscience, #79859) protocols with BODIPY-GDP-loaded KRAS(G12D) concentrated at 0.12 mg/ml and GTP titrated 3-fold from 100 µM - 0 µM.
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl and 1 mM DTT