Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-185
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) with KRAS, Isoform B, His-Tag, GDP-loaded Recombinant (BPS Bioscience #101522) titrated from at various concentrations.
Description: Recombinant human KRAS (KRAS proto-oncogene, GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The recombinant protein (BPS Bioscience #11308) was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT
Genbank: NM_004985
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P01116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
