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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate using Z-Lyte assay conditions.
Description: Recombinant human LCK, GenBank Accession No. NM_005356, full length, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 insect cells using a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT
Genbank: NM_005356
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P06239
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fischer, S. et al. Biochem Biophys Res Commun. 1987 Mar 30,143(3):819-26.
2. Veillette, A. et al. Oncogene Res. 1987 Sep-Oct,1(4):357-74.