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Applications: Useful as a positive control for Western blotting
Description: Mouse LIGHT protein, also known as TNFSF14, GenBank Accession No. NM_019418, a.a. 59-239 with N-terminal His-tag. MW = 22 kDa, expressed in a HEK293 cell expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_019418
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal His-Tag
Uniprot: O43557
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Garrido, V., et al., British Journal of Maematology. 2012; 158(6): 788-797.2.Ogawa, T., et al., Journal of Urology. 2010; 183(3): 1206-1212.
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