Application Note: WB: 1:500. IHC-P: 1:50-1:150. IP: 1:200. ELISA: 1:10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 73
Form: Liquid
Buffer (with preservative): Tris/Glycine, 0.5% BSA, 30% Glycerol, 0.02% Sodium azide.
Concentration: 0.55 mg/ml (Please refer to the vial label for the specific concentration.)
Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]
Uniprot ID: P53667
Antigen Species: Human
Immunogen: Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.
Purification: Purified by affinity chromatography
Conjugation: Unconjugated
Full Name: LIM domain kinase 1
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