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Encompassing Amino Acids: 968-end
Applications: Kinase Assay
Background: LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. Mutations in LRRK2 are the most frequent known cause of autosomal dominant and idiopathic Parkinson's disease with prevalent mutations being found within the GTPase and kinase domains (1). LRRK2 cooperates with MET to promote efficient tumor cell growth and survival in various cancers. Down-regulation of LRRK2 in cultured tumor cells compromises MET activation and selectively reduces downstream MET signaling to mTOR and STAT3 (2).
Description: Human LRRK2 protein with a R1441H mutation, also known as PARK8, RIPK7, and ROCO2, GenBank Accession No. NM_198578, amino acids 968-end, with an N-terminal GST-tag, expressed in an SF9cell expression system. MW = 210 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_198578
Host Cell Line: Sf9 cells
Storage Stability: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Tags: N-terminal GST tag
Uniprot: Q5S007
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yao C., et al., Neurobiol Dis. 2010 Oct;40(1):73-81. 2. Looyenga B., Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1439-44
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