Clone Number: AGR3.4
Immunogen: Purified human AGR3 protein
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.SpecificityThe antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; Positive tissue: human colon.
Western blotting: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
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