Mouse anti-Bromodeoxyuridine, clone IIB5, Purified (Monoclonal)

Clone Number: IIB5

Immunogen: BrdU conjugated to Bovine Serum albumin

Concentration: 1 mg/ml

Storage buffer: PBS with 0.09% sodium azide

Additional info: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorpoRated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorpoRated BrdU with specific anti-BrdU monoclonal antibodies is an accuRate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorpoRated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic prolifeRative information (such as the S-phase transit Rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. IIB5 reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA.The antibody is known to cross-react with Iododeoxyuridine (IdU). Although we have no specific information concerning chlorodeoxyuridine (CldU), it is to be expected that also this antigen is recognized by IIB5.Detection of BrdU incorporated into the DNA needs certain retrieval methods that open up the nucleus and the DNA allow the antibody to reach the antigen. see ref. 1, 5.

References: Schutte et al. Cytometry 1987;8:372-376/Tinnemans et al. Br J Cancer 1993; 67:1217-1222/Schutte et al. Cytometry 1995;21:177-186

• SKU: SANMON8003
• Manufacturer: Sanbio / Monosan
• Manufacturer SKU: MON8003
• Package Unit: 100 µg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Monoclonal
• Application: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Isotype: IgG1
• Host: Mouse
• Conjugate: Unconjugated