Clone Number: 1H7
Immunogen: Purified human placental CD221 IGF-1 receptor
Concentration: n/a
Format: Purified
Storage buffer: PBS with azide
Additional info: IGF-1 Receptor recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (1). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Pretreatment: protein digestion (Trypsin or Pronase) is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended.
References: Li, S.L. et al. (1993) Biochem. Biophys. Res. Commun. 196:92-98./Beauvais, D.M. and Rapraeger, A.C. (2010) J Cell Sci. 2010 Nov 1;123: 3796-807.
English
Deutsch
