Clone Number: B2M-02
Immunogen: Purified isolated human beta2-microglobulin.
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.SpecificityThe mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: liver.
Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred.
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.
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