Clone Number: VU-1D9
Immunogen: Small cell lung carcinoma cell line H69.
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.SpecificityThe mouse monoclonal antibody 6-434 recognizes an extracellular epitope of CD328 (Siglec-7), a 75 kDa transmembrane glycoprotein expressed mainly on NK cells, dendritic cells and monocytes.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 1-4 μg/ml.
Flow cytometry: Recommended dilution: 1-3 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
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