Clone Number: VIM8
Immunogen: THP-1 cell line
Concentration: 1 mg/ml
Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Additional info: CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.SpecificityThe mouse monoclonal antibody B6.2 recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.Application detailsFlow cytometry: Recommended dilution: 1-5 μg/ml.
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