Clone: 15D4.
Background: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.
Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.
Purification Method: The antibody was purified to >95% homogeneity by standard chromatographic techniques.
Source: Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP.
Specificity: Human alpha -SNAP.
Formulation: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been included in the antibody solution.
UniProt: P54920 (Human).
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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