Clone: VU-3C6.
Background: Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.
Positive Control: MCF-7 and T47D cells.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with live human breat cancer cells (cell line ZR-75-1) and mouse myeloma SP2 cells.
Purification Method: Protein A/G Chromatography.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Schol, D.J. et al., Epitope fingerprinting using overlapping 20-mer peptides of the MUC 1 tandem repeat sequence. Tumor Biol. Suppl. 1998: 19, 35-45.
2. Price, M.R. et al., Analysis of 56 monoclonal antibodies against the MUC1 mucin.Tumor Biol. Suppl. 1998: 19, 1-20.
3. Cao, Y. et al., Immunohistochemical characterization of a panel of 56 anti bodies with normal human small intestine, colon, and breast tissues. Tumor Biol. Suppl. 1998: 19, 88-99.
UniProt: P15941.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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