Clone Number: 1027
Immunogen: affinity purified nuclear extract proteins
Concentration: 100 ug/ml
Storage buffer: PBS with 0.02% sodium azide
Additional info: 1027 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
References: Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)/Den Boer, M.L. et al. Blood 91, 2092-2098 (1998)
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