Clone Number: RNL-4
Immunogen: synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C
Concentration: 1 mg/ml
Storage buffer: PBS with 0.09% sodium azide
Additional info: In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
References: Senden et al. Eur J Cell Biol 1996;69:197-213/Senden et al. J Pathol 1997;182:13-21/Hens et al. Cell Tissue Res 1998;292:229-237
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