Clone Number: OPAL1-01
Immunogen: Recombinant fragment of human OPAL1 (amino acids 152-342)
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Additional info: OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.SpecificityThe antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1; intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.
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