Clone Number: dG9
Immunogen: purified granules from human YT lymphoma cell line
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Additional info: Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.SpecificityThe mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.Application detailsFlow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.
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