Clone Number: TRAP3/10
Immunogen: Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.SpecificityThe mouse monoclonal antibody A67-B/E3 reacts with prostate-specific antigen (PSA), a protein produced by normal prostate cells. The highest amounts of PSA are found in the seminal fluid. This antibody recognizes both free and complexed PSA.Application detailsImmunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).
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