Clone: NI 308
Label: HRP
Background: The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
Source: Immunogen: Highly purified tetramethylrhodamine isothiocyanate isomer R.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to tetramethylrhodamine isothiocyanate isomer R
Formulation: Horseradish peroxidase conjugated purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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