Storage: -20℃,12 months
Abbreviation: CD117
Target Synonym: KIT proto-oncogene,receptor tyrosine kinase,PBT,SCFR,C-Kit,CD117,MASTC
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse CD117/c-kit concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Type: Sandwich-ELISA
Detection Type: Colorimetric method;ELISA;Sandwich
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD117/c-kit . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD117/c-kit and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD117/c-kit , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CD117/c-kit . You can calculate the concentration of CD117/c-kit in the samples by comparing the OD of the samples to the standard curve.
Cross_reactivity: Detects Mouse CD117/c-kit in ELISAs
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