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Amino Acid Sequence: APT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL NEEVEVVSNE FSFKKLTCVQ TRLKIFEQGL RGNFTKLKGA LNMTASYYQT YCPPTPETDC ETQVTTYADF IDSLKTFLTD IPFECKKPGQ K
Background: GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the N-terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.1ng/ml, corresponding to a specific activity of≥ 3 x 10^6 units/mg.
Description: Recombinant mouse GM-CSF is a disulfide-linked monomer protein, consisting of 125 amino acids and migrates as an approximately 14 kDa protein under reducing conditions. Optimized DNA sequence encoding murine Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Genbank: P01587
Purity: ≥95% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P01587
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hiasa, M., et al. Blood November 12, 2009 vol. 114 (20): 4517-4526 .
2. Zhu, S.M., et al. J. Exp. Med. 206 (10): 2141-2149.
3. Chin, P.Y., et al. Hum. Reprod. (2009) 24 (12): 2997-3009.
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