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Applications: Useful for the study of mouse IDO2 enzymology, screening inhibitors, and selectivity profiling.
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for proteinsynthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established asa key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulatedin many tumors, providing cancer cells with an avenue for immune evasion.
Contraindications: DMSO >0.5%, strong acids or bases, ionic detergents, high salt
Description: The IDO2 Inhibitor Screening Assay Kit is designed to measure mouse IDO2enzyme inhibition. The Mouse IDO2 Inhibitor Screening Assay Kit issimple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After aroom temperature incubation, activity is determined by measuring the absorption of reactionproduct at l=320 - 325 nm.BACKGROUND: L-tryptophan
Storage Stability: At least 6 months from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient format with enough reaction solution and enzyme to perform a total of 100 reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J. Biomol. Screen. 2014; 19(9): 1266-74.
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