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Encompassing Amino Acids: 530-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Add 50 µl reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2 mM ATP) to wells coated with substrate. Incubate for 1 hour. Add Europium-labeled anti p-Tyr antibody and incubate for 1 hour. Time resolved fluorescence measured at room temperature at λexc=330+/-40 nm and λem=620+/-10 nm.
Description: Human MUSK (GenBank Accession No. NM_005592), a.a. 530-end with N-terminal His-tag, MW = 38.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
Genbank: NM_005592
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: O15146 Q13255
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhou H. et al., J. Cell Biol. 146 (5), 1133-1146 (1999).
2. Evoli A. et al., Ann. N. Y. Acad. Sci. 1132, 76-83 (2008).
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