Shelf life (days): 180
Notes: The Naveni PD1/PD-L1 kit is an in situ proximity ligation assay kit for the detection of the interaction of PD1 and PD-L1. This in situ kit gives a high signal-to-noise ratio and has an out-standing specificity. The kit is comatible with immunofluorescence co-staining for visualization of the tumor microenvironment. The kit has been validated on FFPE human tissue samples and tested in fresh frozen tissue. Not recommend for mouse tissue. The kit is based on our Naveni™ Proximity Ligation Technology, with TriFlex Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection; learn more about Naveni technology. One Navenibody binds the PD1 protein, and the other Navenibody binds PD-L1 . Probes in close proximity will give a strong and distinct signal, creating a specific and sensitive detection method for the interaction between PD1 and PD-L1. Included in the kit: • Navenibody targeting human PD1 protein based on clone EH33 CST • Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb® • Detection fluorophore; Atto647N • Buffers for blocking and dilutions, and all detection reagents • The Navenibody solution has a working volume of 4000 µl (sufficient for approximately 100 reactions) Not included in the kit: • Tris EDTA antigen retrieval buffer, pH 9.0 • Wash buffer • DAPI nuclear stain • Mounting medium Also available with HRP Substrate (Item No. 39216) or AP Substrate (Item No. 39215) for bright-field readout.
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