Target: NCC.
Conjugate: RPE .
Product Type: Polyclonal.
Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal.
Swiss-Prot: P55018, P55017 .
Purification: Affinity Purified.
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide.
Concentration: 1 mg/ml.
Specificity: Detects ~160kDa.
Cellular Localization: Membrane.
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
References: 1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712.2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562.3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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