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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: NDR or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family that is regulated by Mob proteins (1). Fas and TNFα receptor stimulation activates human NDR by promoting phosphorylation at the hydrophobic motif. NDR acts as a novel pro-apoptotic kinase, and is a key member of the RASSF1A/MST1 signaling cascade (2). NDR is crucial for the fidelity of mitotic chromosome alignment in mammalian cells. NDR is incorporated into the HIV-1 particles and is cleaved by the HIV-1 protease. NDR-driven centrosome duplication requires Cdk2 activity, and Cdk2-induced centrosome amplification is affected upon reduction of NDR activity.
Description: Recombinant human NDR, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_007271
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q15208
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Millward, T. et.al: Molecular cloning and characterization of a conserved nuclear serine (threonine) protein kinase. Proc. Nat. Acad. Sci. 92: 5022-5026, 1995.
2. Vichalkovski A. et al: NDR kinase is activated by RASSF1A/MST1 in response to Fas receptor stimulation and promotes apoptosis. Curr Biol. 2008 Dec 9;18(23):1889-95.
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