Application Note: This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 µl per assay. This product is tested for immunohistochemistry in human thymus tissue at 1:500.
Concentration Value: 90 mg/mL
ELISA Dilution: 1:5,000 - 1:25,000
Gel Shift Dilution: 0.5 to 1.0 µL
Immunohistochemistry Dilution: 1:500
Western Blot Dilution: 1:500 - 1:3,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical State: Liquid (sterile filtered)
Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination.
Background: Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Low Endotoxin: No
Other: User Optimized
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