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Encompassing Amino Acids: 2 - 1203 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human nitric oxide synthase 3 (NOS3), also known as endothelial NOS (eNOS), (GenBank Accession No. NM_000603), a.a. 2 - 1203 (end) with N-terminal His-FLAG-tag, MW= 135 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 260 mM imidazole, and 20% glycerol
Genbank: NM_000603
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tag
Uniprot: P29474
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Marsden, P.A., et al., FEBS Lett. 1992 Aug 3;307(3):287-93.
2. Liu, Y., Feng, Q., Differentiation. 2012 Jul;84(1):54-61.