Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 1849-2094
Applications: Suitable for protein binding studies, as an immunogen, or as a Western blotting control.
Assay Conditions: Assay wasperformed using the BPS NSD1Chemiluminescent Assay Kit (Cat#53010) with 400 µM S-adenosyl-methionine on biotinylated H3 peptidecoated plates overnight at roomtemperature.
Description: Human NSD1 (GenBank Accession No. NM_022455), amino-acids 1849-2094 with N-terminal GST tag, MW = 54 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mMGlutathione, 20% Glycerol and 3mM DTT.
Genbank: NM_022455
Host Cell Line: E. coli
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: Q96L73
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yamada-Okabe, T. and Matsumoto, N.J. Cell Biochem. Funct. 26 (2), 146-150 (2008).
2. Saugier-Veber, P. et al., Hum. Mutat. 28 (11), 1098-1107 (2007).Application Reference(s):
A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014)