OneStep PLUS qMethyl PCR Kit

Use the promocode EPI20 until December 24th 2024 on your order to get a 20% discount on our research tools for discovering, exploring & mapping the epigenetic landscape! No additional discounts can be applied during the promotion period.

Description: The OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite-free procedure for the quantification of DNA methylation at custom-selected genomic regions. This bisulfite-free assessment is accomplished by splitting the DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both reactions is then amplified using Real-Time PCR. The difference in Cycle Threshold (Ct) values between the Reference and Test reactions is used to calculate the percentage of DNA methylation at the selected genomic region..

• No bisulfite conversion needed: from DNA sample to results in just one step.
• Accurate quantification of DNA methylation levels at the genomic regions of choice.
• Ideal for rapid screening of DNA methylation at single and multi-locus.

• SKU: ZYMD5312
• Manufacturer: Zymo Research
• Manufacturer SKU: D5312
• Package Unit: 1 Stück
• Quantity Unit: STK