Application Note: Anti-ORAI-1 has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 32.7 kDa by western blotting in the appropriate cell lysate or extract.
Concentration Value: 45 mg/mL
Western Blot Dilution: 1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-ORAI-1 antibody is directed against the human ORAI-1. The product was prepared from monospecific antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography. Cross-reactivity with ORAI1 from other sources have not been determined.
Background: The ORAI-1 gene codes for the calcium release-activated calcium (CRAC) channel subunit in humans. The CRAC channel subunit mediates calcium influx when activated by the calcium sensor, STIM1, following depletion of intracellular calcium stores. CRAC channels are the primary pathway for calcium influx in T-cells and promote the immune response to pathogens through activation of the transcription factor NFAT. Defects in this gene can be a cause of immune dysfunction due to inactivation of T-cells.
Low Endotoxin: No
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