Application Note: This product was tested by immunoblot and found to be reactive against p16 from cell lysates and E.coli produced fusion proteins at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (code #611-1302). Anti-p16 is suitable for the detection by immunoblot of human p16. This antisera is also suitable for immunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5. This product was tested in IHC.
Concentration Value: 85 mg/mL
ELISA Dilution: 1:5,000 - 1:20,000
Immunohistochemistry Dilution: 1:500
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: human p16 protein (missing the first 7 amino acids)
Physical State: Liquid (sterile filtered)
Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a p16 protein from human tissue. Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots. Potentially cross reacts with p15.5.
Background: The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Low Endotoxin: No
Other: User Optimized
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