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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: p70S6K is responsible for the phosphorylation of the 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. In intact cells p70S6K is activated by serum and this activation is inhibited by wortmannin and rapamycin. p70S6k activity is cell cycle-dependent and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylation of serine residues in the putative auto-inhibitory pseudo-substrate domain, of threonine 389 phosphorylated by mTOR, and of threonine 229 phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The P70S6K (S6K1) Kinase Assay Kit is designed to measure P70S6K (S6K1) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant P70S6K (S6K1) kinase, kinase substrate, ATP and Kinase Assay Buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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