Clone: PASE/4LJ.
Background: The antibody against PAP may be used for the immunohistochemical detection of PAP in normal and diseased prostate and in metastases thereof. Because of the limited specificity of the enzyme, the antibody should only be used in parallel with specific antibodies like PSA (Art. No. PS100 and PS500) , P504S, P501S, ERG and other cancer-specific markers. Prostata Saure Phosphatase, PAP (E.C.3.1.3.2) ist ein Isoenzym der Sauren Phosphatase und wird in großen Mengen in Prostataepithel und in Samenflüssigkeit gefunden. Es ist ein homodimeres Protein aus zwei ca. 50 kDa Untereinheiten. Außer in der Prostata wird PAP auch anderen Geweben, wie in Pankreas, Colon, Blase, Lunge und in der Niere sowie in Karzinomen der Brust, Ovar und Colon gebildet, allerdings in deutlich geringeren Mengen (3). Human PAP, 48 kDa subunit, no cross reactivity with PSA.
Positive Control: Prostate carcinoma.
Immunogen: Purified human Prostate Acid Phosphatase from seminal plasma.
Purification Method: Purified antibody in PBS containing BSA and 15mM NaN3.
References: 1. Sharief FS, Lee H, Leuderman MM, Lundwall A, Deaven LL, Lee CL, Li SS (1989) Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase. Biochem Biophys Res Commun. Apr 14;160(1):79-86. 2. Veeramani S, Yuan TC, Chen SJ, Lin FF, Petersen JE, Shaheduzzaman S, Srivastava S, MacDonald RG, Lin MF (2005) Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer. Dec;12(4):805-22. 3. Graddis TJ, McMahan CJ, Tamman J, Page KJ, Trager JB (2011) Prostatic acid phosphatase expression in human tissues. Int J Clin Exp Pathol. Mar;4(3):295-306. Epub 2011 Mar 22. 4. Queisser A., Hagedorn S.A., Braun M., Vogel W., Duensing S., Perner S. (2015) Comparison of different prostatic markers in lymph node and distant metastases of prostate cancer. ModernPathology 28, 138–145.
UniProt: P15309 (PPAP_HUMAN).
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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