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Encompassing Amino Acids: 782-872
Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Description: Human polybromo 1 (PB1), also known as PBRM1 and BRG1-associated factor 180, variant 2, (GenBank Accession No. NM_018313), a.a. 782-872 corresponding to bromodomain 6 with N-terminal His-tag, MW = 12 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 400 mM imidazole and 20% glycerol.
Genbank: NM_018313
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q86U86
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sekine, I., et al., Oncogene. 2005 Apr 14;24(16):2735-8.
2. Shibata, Y., et al., Dev Biol. 2012 Jan 15;361(2):349-57.
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