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Encompassing Amino Acids: 25-167
Applications: Useful for labeling PD-L1 expressing cells for flow cytometry or immunohistochemistry.
Description: Mouse secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_008798, a.a 25-167, fused at the C-terminus to the Fc portion of mouse IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Genbank: NM_008798
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: Fc Fusion, PE Labeled
Uniprot: Q02242
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1. Agata, A., et al., Immunol. 1996 Oct;8(5):765-772.2. Freeman, G., et al., JEM. 2000 Oct; 192(7):1027-1034.