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Applications: This kit is useful for screening for inhibitors of mouse PD-L1 binding to mouse B7-1.
Background: B7-1, also known as CD80, is a membrane receptor found in activated antigen presenting cells (APC). In addition to PD-1, PD-L1 is able to bind to B7-1, resulting in inhibition of T cell activation and cytokine production. This immune checkpoint is another target of interest for immunoregulatory functions in oncology and other disease areas. Mouse PD-L1 and B7-1 are the respective orthologs of the human proteins, so they can be used for research in animal models.
Description: The PD-L1:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (Mouse) is designed for screening and profiling inhibitors of murine PD-L1 binding to murine B7-1. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, biotinylated B7-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Butte, M. J., et al. mmunity 2007, 27(1):111-22 Chaudhri, A., et al. Cancer Immunol Res 2018, 6(8): 921-929
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